|Hair Color||Pale Blue|
|Novel Debut||Volume 1|
|Anime Debut||Episode 01|
Reine Murasame (村雨 令音 Murasame Reine?) is the Head Analyst aboard Fraxinus and a non-certified medic who can perform simple first aid tasks. On the second day of the semester, she transfers into Shido's school as his assistant homeroom teacher.
Reine is a highly intelligent and rational woman. She is the Analysis Officer of the Fraxinus’s crew and the Ratatoskr's organization, and the only member that could be considered "normal" among the Fraxinus's weird crew. Reine also acts as an unofficial aide to Kotori Itsuka, the commander of Fraxinus, due to the fact that the vice-commander acts like an idiot most of the time or is ignored by Kotori for being an idiot.
Beautiful and intelligent, her only flaw is being sleep deprived, since she doesn't seem to sleep much. Reine transfers into Shido’s school, Raizen High School as the school's new physics teacher when Shido joined Ratatoskr. Reine usually gives the best advice or opinions out of Ratatoskr's crew during both the Fraxinus crew's operations to seal Spirits and normal circumstances.
Reine is unarguably a beautiful woman with pale skin. She still looks to be in her 20s, despite saying that she hasn't slept in 30 years. She has long, unkempt, pale blue hair which is tied into a right-side ponytail and blue eyes which seems to always be sleepy. Her most distinct feature is the pair of dark circles beneath her eyes. She wears a pair of glasses with a circular frame. Her height is 164 cm, and her three sizes are B95/W63/H89.
She is usually or always seen with a small blue teddy bear that fits in her breast pocket. During her duty on Fraxinus, she wears <Ratatoskr>'s uniform, while as a Physics teacher at Shido's school, she wears a long white lab coat over a pink shirt with a rather low V-neck collar, purple miniskirt, a pair of black stockings, and a pair of blue teddy bear slippers. She also wears a small silver ring on her silver chain necklace.
Reine is a very logical, reasonable, and mature woman. Surprisingly enough, and despite her usual cold demeanor, she has proven countless times to be a very sensitive, considerate and understanding person.
She is quick witted enough to adapt to the sudden appearance of the Yamai sisters and seamlessly integrate them into her class field trip without raising suspicions from others. She's also good at giving advice and coaching Shido during his dates with the Spirits, even Kotori seems to take her advice well.
Reine is very good with handling children as when Natsumi turned everyone but Shido into children she was able to calm them all instantly. She has also displayed this skill towards Shido himself, calming him down after he recognized Kotori as a Spirit. Shido himself stated that she is like a mother, which she seems to react to in a strange way.
While Reine is normally calm and collected, there are rare occasions where she shows anger. These instances are rare enough that Kotori had never seen her angry until the events of Volume 3, when Reine showed her Mana's body had been heavily modified to give her an abnormal strength at the cost of lowering her remaining lifespan to 10 years.
- "...But, what I want to hear is not that emotional kind of reason.......It's troublesome when you play dumb. I won't believe that you don't understand. —What exactly is he?" 
- "……But, we have to be careful. LoveThat could also be, the emotion that will destroy the world." 
- She has a tendency to faint suddenly due to her suffering from sleep deprivation.
- She cannot pronounce Shido's name, which is why she calls him Shin.
- The kanji for "zero" (零) can be created with the first kanji of her name (令) plus the second kanji of her surname (雨).
- In the Kaballah, Zero stands for the three mysterious forces located above, and said to be the origin of, the Kaballah's Tree of Life, "En" (Nothingness), En Sof (The Infinite), and "Ohr En Sof" (The Infinite Light).
- This leads to a lot of fans speculating that she is either Phantom or the First Spirit.
- Phantom's speech pattern seems similar to Reine's.
- Reine's Voice Actor, Aya Endo, also voices Phantom in Date A Live: Rinne Utopia.
- When Kotori mentioned Shido was near to being suicidal in the past in Light Novel Volume 1, Reine reacted strangely to this, almost emotional.
- She also was noticeably angry when she saw the modifications that had been made on Mana's body.
- She also gave a similar reaction when Shido mentioned she was like a mother after she calmed other Spirits whom Natsumi made younger.
- When Reine and Origami were attacked during the events of Light Novel Volume 5, she claimed that Origami had saved her but Origami had passed out during the time when the Bandersnatch was about to attack. When Origami regained consciousness, the Bandersnatch was deliberately in pieces and both Origami and Reine were unharmed.
- She has told Shido at the beginning of the series, "I haven't had a decent sleep in 30 years", which oddly enough is around the same time the first spacequake was caused by the First Spirit which began the start of the series itself.
- In the English Dub, Reine's name is pronounced as 'Reina'.